DNA damage induced by radiation exposure from cardiac catheterization – an analysis in patients and operators
نویسندگان
چکیده
Abstract Background The biological effects of low-dose radiation from cardiac imaging or intervention remain largely unknown. This study aimed to evaluate the ionized catheterization on genomic DNA integrity and inflammatory cytokines in patients operators. Methods Peripheral mononuclear cells (MNCs) were isolated (n=52) operators (n=35) before after coronary angiography and/or percutaneous intervention. Expression gammaH2AX, a marker for double-strand breaks, was measured by immunofluorescence. Dicentric chromosomes (DICs), form chromosome aberrations, assayed using fluorescent situ hybridization technique. Results In patient MNCs, numbers gammaH2AX foci DICs increased 101±75% 28±99%, respectively (P<0.05 both). mRNA expressions interleukin (IL)-1α, IL-1β, leukemia inhibitory factor (LIF) caspase-1 significantly exposure catheterization. increase IL-1β correlated with that but not dose area product. operators, neither nor level changed, increased. Protein expression IkappaBα decreased both groups. Conclusions damage MNCs patients, who underwent Inflammatory presumably through activation NF-kappaB. Further efforts reduce are necessary Funding Acknowledgement Type funding sources: Public grant(s) – National budget only. Main source(s): Grants-in-Aid Scientific Research (KAKENHI)
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ژورنال
عنوان ژورنال: European Heart Journal
سال: 2022
ISSN: ['2634-3916']
DOI: https://doi.org/10.1093/eurheartj/ehac544.3015